Abstract
Bacteriophages can be used as biocontrol agents to eliminate the undesirable and targeted
bacteria in human beings, environment and industries. We aimed to isolate phages from cow manure and
investigate their antibacterial and anti-biofilm formation effect against MRSA and E. coli species.
Methods: E. coli and S. aureus isolates of clinical origin were sub-cultured on MacConkey’s and Mannitol salt
agar media, respectively. To confirm the Methicillin resistance in S. aureus, mecA gene was detected by the
PCR using gene specific forward and reverse primers. The bacteriophages were isolated using enrichment
procedure from cow manure. Their antibacterial and anti-biofilm formation activity was determined through the
inhibition of bacterial growth and decrease in the absorbance of Crystal Violet through biofilm inhibition assay,
respectively.
Nimerta Kumari, Bushra Bano Patoli, Atif A Patoli, Shagufta Jabeen. (2019) Biocontrol of MRSA and E. coli using bacteriophages from cow manure, Advancements in Life Sciences, Volume 7, Issue 4.
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