Abstract
Background: β - thalassemia major is inherited disorder of single gene defect that occur as a result of more than
200 mutations in the beta goblin gene causing its impaired synthesis. Frequent blood transfusions are necessary
for the treatment and management of thalassemia major patients. These excessive transfusions can often lead to
iron overload that can damage many organs including liver, endocrine glands and spleen leading to lifethreatening complications. This study was conducted to estimate the variables like iron ferritin and
aminotransferases (ALT, AST) in serum and saliva of β-thalassemia patients.
Materials and methods: This study was conducted during a year from January-December 2018. It was a cross
sectional comparative study. Forty diagnosed cases of β-thalassemia major were included in the study. From each
patient both serum and saliva samples were collected. The levels of ALT and AST in serum and saliva samples of
all study subjects were measured on chemistry analyzer using enzymatic kinetic method. Iron levels were
measured by using ferrozine chromogenic method and absorbance were taken on microplate reader (Bio-RAD
analyzer) capability and serum and saliva ferritin was measured by enzyme-linked immune-sorbent assay
(ELISA) method.
Results: The results of the study showed a statistically significant difference between these two groups whereas
the correlation analysis showed moderate correlation between serum and saliva iron and AST levels, weak
correlation between serum and saliva ferritin level while strong correlation between serum and saliva ALT level.
Conclusion: This study concludes that saliva cannot be used as diagnostic marker for iron overload and liver
damage in β -thalassemia major patients however saliva can be used as predictive marker.
Anam Qaiser, Shifa Azia, Naila Irum, Sunbla Ghaznavi. (2020) Comparison of Levels of Iron, Ferritin and Aminotransferases in Serum and Saliva of Beta Thalassemia Major Patients, International Journal of Pathology, Volume-18, Issue-3.
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