Abstract
This study was planned for the production of lipases by solid-state fermentation (SSF) using agro-industrial wastes as substrate. A total of 14 bacterial strains were isolated, screened and compared for lipase production. Among the evaluated strains, Bacillus subtilis strain NL-39 was selected on the basis of best lipase production. The maximum
enzyme activity (34.93 U/ml) was shown by B. subtilis using SSF with soya bean meal. The lipase production by other low cost agro-industrial waste, including sunflower meal, wheat bran, oat bran, rice bran and sugar cane bagasse was 23.83, 12.17, 10.40 10.00 and 16.23 U/ml, respectively. The lipase production by B. subtilis using peels
of different fruits, including banana, orange, water melon and melon as carbon source, was 27.17, 21.37, 10.57 and 8.43 U/ml, respectively. The corn cob produced 12.27 U/ml while waste oils of various industries produced 16.17 U/ml (Shan oil), 13.67 U/ml (automobile), 13.37 U/ml (unbranded waste cooking oil) and 6.03 U/ml (Sitara oil) of
enzyme activity when used as substrate for lipase production. It is concluded that among the agro-industrial wastes, fruit peels and waste oil samples used in this study, the maximum lipase production was observed with soya bean meal, banana peel and Shan oil waste, respectively.
Haniya Mazhar, Naaz Abbas, Zahid Hussain, Syed Shahid Ali, Amir Sohail. (2016) Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels, Punjab University Journal of Zoology, Volume 31, Issue 2.
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