Abstract
In the present work, two marine gastropods (Nerita albicilla and N.
undata) were subjected to detailed investigation highlighting the biomass
distribution, phenotypic and genotypic identification, biochemical
composition including total lipid, carbohydrate, protein, fatty acid and
amino acid profiles. The sampling was done at Paradise Point and Buleji
located at the Sindh Coast of Pakistan. Microstructure and chemistry of
the shell was examined via Scanning Electron Microscopy (SEM) and
Energy Dispersive X-ray Spectrometry (EDX). The biochemical
composition of Nerita sp. showed high concentration of proteins followed
by lipids and carbohydrates. Hemocyanins, the medicinally important
protein was abundantly found in the hemolymph of both species and was
confirmed by SEC FPLC and SDS-PAGE analysis. Fatty acid profile
showed eight different types of fatty acids, while amino acid analysis
showed rich composition of non-essential and essential amino acids. Our
results present successful method of identification at genetic level by
amplification of mitochondrial gene 16SrDNA. The data from current
study also exhibit nutritionally rich status of Nerita sp. which can be
utilized as significant and valuable marine resource of high energy and
can also be an alternative source of balanced diet (food/feed) to
overcome the problem of malnutrition in underdeveloped countries.
FOZIA HUMAYUN, IQRA MUNIR, MUHAMMAD SAMEE HAIDER, ZARRIEN AYUB, SYED ABID ALI. (2020) The molecular and biochemical characterization of two Marine Gastropods of the Genus Nerita, Biologia – Journal of Biological Society of Pakistan, Volume 66(I), Issue 1.
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