Abstract
New antimicrobials agents are needed to combat drugs resistance in
microorganisms. The aqueous and methanolic extracts of Nigella
sativa and Ficus carica seeds were used against bacteria
(Staphylococcus aureus and Escherichia coli) and Fungi (Candida
albicans, Fusarium oxysporium and Microsporum canis) to check their
antimicrobial activities. For antibacterial assays, the volume and
concentrations of extracts used were 0.5 µl (62.5 µg), 1 µl (125 µg), 5 µl
(625 µg) and 10 µl (1250 µg). Specific laboratory protocols of antibacterial
and antifungal assays were adopted. Absorbance of the broth bacterial
culture was measured after 72 hours by using photo spectrometer at the
wavelength of 600 nm (A600). For antifungal assays, the volume and
concentrations of extracts used were 2 µl (250µg), 4µl (500µg), 8 µl (1000
µg) and 10 µl (1250 µg). The methanolic extract of Nigella sativa (1µl,
125µg) and water extract of Ficus carica (1µl, 125µg) were found most
effective against E. coli (A600 0.003) whereas, water extract of Ficus
carica (1µl, 125µg) was found most effective against Staphylococcus
aureus (A600 0.003). Nigella sativa methanolic extract (8 µl, 1000µg) was
found most effective against Candida albicans and Fusarium oxysporum
with inhibited fungal growth zone diameter of 15mm, while Ficus
carica methanolic extract (10 µl, 1250 µg) was found most effective
against Fusarium oxysporum and Microsporum canis with diameter of
inhibited fungal growth zone 19 mm and 21 mm, respectively. These
experiments showed that these extracts can be used as antibacterial
agents
SHAHZADA NADEEM ABBAS, KIRAN JAMSHED, SARAH BUSHRA NASIR, SAMAN SHAHID , NASIR MAHMOOD. (2019) Antimicrobial activities of Methanolic and Aqueous extracts of Nigella sativa and Ficus carica, Biologia – Journal of Biological Society of Pakistan, Volume 65 (I), Issue 1.
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