Abstract
The degradation of RNA is main hindrance for dealing with fine quality
RNA for its downstream processing. Mostly RNAs are degraded as
tissues are taken from fields, therefore the protocol for PSTVd RNA
isolation was optimized and improved for obtaining Pure and high quality
RNA having an increased and nondegradable life from infected plants of
Potatoes. The present study was aimed to confirm the presence and
identification of potato spindle tuber viroid (PSTVd) on potato plants in
Pakistan. RNA was extracted and purified using optimized Trizol reagent
and RNeasy Miniplant Kit. The RNA obtained was converted into cDNA
on the same day and amplified. The identification of PSTVd was carried
out by Sequencing and BLAST. The advanced optimized Rapid Trizol
reagent method was comparatively found best and reproducible as
compared to the second method. The Sequencing and BLAST confirmed
the presence of Pakistani PSTVd (Accession # MK.303578) in infected
potato plants.
ZARYAB KHALID SIAL, FARAH KHAN. (2018) First report on Potato Spindle Tuber Viroid (PSTVd) from field grown infected Potato plants (Solanum tuberosum) in Pakistan, Biologia – Journal of Biological Society of Pakistan, Volume 64 (II), Volume 64 (II).
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