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Objectives: Evaluate the antioxidant effects of Virgin Coconut Oil and Corn Oil in Phenytoin induced toxicity of seminiferous tubules in rats. Study Design: Experimental study. Setting: Al-Tibri Medical College and Hospital, Period: November 2019 to May 2020. Material & Methods: 48 male albino rats (weighing between 150-250gms) were selected based on a randomized sampling technique and divided into four groups, 12 rats per group and euthanized on the 4th, 5th, and 6th week of the study. Group A control, B was given Phenytoin, C received phenytoin +virgin coconut oil, and D received Phenytoin + corn oil once daily for six weeks. The sample of sperm was taken from epididymal fluid and counted by using the Neubauer chamber. The data was analyzed through SPSS version 21.0. Mean values were compared through the application of One way ANOVA followed by post hoc Tukey’s test. P-value was considered significant <0.05. Results: In Group B, there was a significant decrease in spermatozoa count due to Phenytoin induced toxicity. At the same time, there was a considerable restoration in the number of spermatozoa in group C compared to D. Conclusion: In Group B, there was a significant decrease in spermatozoa count due to Phenytoin induced toxicity of seminiferous tubules, and those animals received the virgin coconut oil along with phenytoin help in the restoration of numbers of spermatozoa as compared to group D. Virgin coconut oil showed significant antioxidant role in comparison with corn oil.

Khalique ur Rehman, Hina Khan, Shahid Hussain Soomro, Asad Raza Jiskani, Raja Faisal, Farheen Hameed. (2020) Restoration of spermatozoa: Antioxidant effects of virgin coconut oil and corn oil in Phenytoin induced toxicity of seminiferous tubules in rats., The Professional Medical Journal, Volume 27, Issue 12.
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