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Aim of the present study was to investigate the status of different antioxidant systems in semen and peripheral blood of bulls of
two age groups. For this purpose, different physiological biomarkers in the peripheral blood and seminal plasma of 3-5 years
old and 8-10 years old Sahiwal bulls, kept at the Semen Production Unit (SPU), Qadirabad, Sahiwal were monitored. Six bulls
with clinically normal reproductive tract were included in each group. Semen samples were collected from each bull weekly
for four weeks, as per routine practice at the SPU, and examined for semen quality parameters. Then seminal plasma was
separated by centrifugation and stored at -20ºC. On the day of semen collection, blood was also collected from each bull, serum
was separated and stored at -20ºC. Serum and seminal plasma samples were thawed and analyzed for physiological biomarkers
including total antioxidant status (TAS), total oxidant status (TOS), paraoxonase, arylesterase, malondialdehyde (MDA),
ceruloplasmin and catalase. Results showed that mass activity, individual sperm motility and sperm concentration were
significantly higher (P<0.05) in 3-5 years old bulls than those in 8-10 years old bulls. However, dead sperm percentage did not
differ between 3-5 year (12.54±0.25%) and 8-10 year (12.75±0.33%) old bulls. Serum activities of paraoxonase, TAS and TOS
were significantly higher (P<0.05) in 3-5 years old than 8-10 years old bulls, while reverse was true for serum ceruloplasmin
activity. However, serum MDA, catalase and arylesterase activities did not differ significantly between bulls of two age groups.
Seminal plasma showed higher activities of paraoxonase, arylesterase and ceruloplasmin in 8-10 years old bulls compared to
bulls aged 3-5 years (P<0.05), while an opposite trend was seen for TAS and TOS. Moreover, bulls aged 3-5 years had higher
activities of paraoxonse and arylesterase in the serum, while older bulls showed higher activities of paraoxonse, arylesterase,
TAS, and ceruloplasmin in the seminal plasma. Activities of catalase and MDA were higher in the serum than in seminal
plasma in bulls of both age groups (P<0.05). However, activities of TAS in bulls aged 3-5 years and TOS in older bulls did not
differ between the two body fluids. In conclusion, semen quality in terms of mass activity, individual sperm motility and sperm
concentration was better in 3-5 years old compared to 8-10 years old Sahiwal bulls. However, no specific trend for levels of
various physiological biomarkers in relation to semen quality of these bulls could be demonstrated
Irfan Ahmad, Ijaz Ahmad, Nazir Ahmad, Zafar Iqbal Qureshi, Huma Jamil, Zia-ur-Rahman. (2020) DETERMINATION OF PHYSIOLOGICAL BIOMARKERS IN THE SEMEN AND PERIPHERAL BLOOD OF SAHIWAL BULLS OF TWO AGE GROUPS, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 1.
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