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Bacteriocins are the antimicrobial peptides that are produced by many of the bacterial species as secondary metabolites. They
have potential to inhibit the growth of many other pathogenic bacteria. The present study was conducted to check production
and therapeutic potential of bacteriocin produced by different indigenous isolates of Bacillus subtilis. Soil samples were
collected from different environments including soil mud, agricultural land soil, sewage, and deteriorating plant matter. These
samples were cultured on Nutrient Agar for isolation of B. subtilis. These isolates were further purified by culturing and
identified by biochemical.16SrDNA technique was used to confirm the B. subtilis. The confirmed isolates were evaluated for
their potential to produce bacteriocin using agar well diffusion method. Bacteriocin was purified using Ammonium sulfate
precipitation method, and its chemotherapeutic properties were checked by developing two models, Superficial skin model and
experimental Gram negative septicemia model. The results indicated that B. subtilis isolates showed a band of 1311bp on gel
in 16SrDNA analysis and sequencing also confirmed B. subtilis isolates. No toxic effect of bacteriocin was observed in
experimental animals. There was no mortality in treated and controlled group animals during experimentation. In conclusion,
bacteriocin peptides have ability to inhibit bacterial growth and may be exploited to treat microbial infections.
Riffat Shamim Aslam, Muhammad Ashraf, Mashkoor Mohsin, Zafar Iqbal. (2020) PRODUCTION AND THERAPEUTIC POTENTIAL OF BACTERIOCIN PRODUCED BY INDIGENOUS ISOLATES OF Bacillus subtilis, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 5.
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