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Rose is a highly economically important ornamental plant being globally used for aesthetic purposes since ancient times. In
Pakistan, during last seventy years hundreds of Hybrid varieties were introduced and adopted for cut-flower production and
landscape beauty. Several spontaneous mutants may also have originated during clonal propagation. In 2008, a comprehensive
survey of Punjab areas was conducted and several hybrids were collected and planted in semi sub-tropical area
(Rawalpindi/Islamabad). The varieties with stable phenotypic characters were further subjected to morphological analysis
following UPOV-Rose descriptors during 2017 to 2019. Morphological diversity was analyzed using principal component
analysis (PCA), cluster analysis and a large ratio of the characters was considered as dominant. The narrow range of variation
was found within germplasm of Hybrid accessions and traits (11.43% and 10.14%) on factor plains (Factor 1 × Factor 2). The
leading accessions 33, 34 and 6 were selected for their desirable plant attributes such as better flower size (cm), stem length
(cm) and early flower production. Establishment of in vitro clonal propagation was required for efficient multiplication. The
explants were surface sterilized by pre-soaking in Topsin-M fungicide and sequential use of HgCl2 and NaOCl. Phenolic
exudation was controlled using 1% Citric acid solution. Among selected accessions, #33 performed better for efficient
establishment of cultures and in vitro clonal multiplication. Hence, it may be used for further clonal propagation using tissue
culture for commercial production. This is first report of the adoptability studies of different HT-Rose accessions in Pakistan
and their capacity to be used in selection and breeding programs.
Monis Hussain Shah, Riaz Ur Rahman, Abid Mahmood, Muhammad Usman, Sajida Bibi. (2021) MORPHOLOGICAL CHARACTERIZATION, MULTIVARIATE ANALYSIS AND MICROPROPAGATION OF HYBRID ROSE (Rosa indica L.) GERMPLASM, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 1.
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