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The present study was conducted to evaluate the effects of limiting amino acids supplementation in low crude proteins diet on
Labeo rohita fingerlings having an average weight of 4.340g. Fish were kept in n hapas and were fed two times a day @ 3%
of body weight to obvious satiation at 10 AM and4:00 PM regularly for ninety days in triplicates on five diets designated as,
D1 (30% CP and NRC recommended amino acid level) as control diet, D2 (with 2% low protein and 5% amino acid
supplementation), D3 (with 2% low protein and 10 % amino acid supplementation), D4 (with 4% low protein and 10 % amino
acid supplementation) and D5 (with 4% low protein and 20 % amino acid supplementation). Net increase in weight, specific
growth rate and percent increase in weight were significantly increased while significantly lower value of feed conversion ratio
was observed in regimes served with D5 diet. However, meat proximate composition showed increase in protein level with
significant decreased in lipid content at 4% low protein and 20 % amino acid supplementation in diet. Furthermore, erythrocytes
increased with the increased level of limiting amino acids supplementation in diet among treatments. Whole body essential
and non-essential amino acids content, showed significant increase values with the addition of limiting amino acids among the
treatments. Consequently, Labeo rohita fingerlings showed positive response to limiting amino acids supplemented diet in low
crude protein. Therefore, such diets can be used safely in fish feed to decrease the cost of production in aquaculture.
Amina Ayub, Fayyaz Rasool, Noor Khan, Shafqat Nawaz Qaisrani, Shakeela Parveen. (2021) LIMITING AMINO ACIDS SUPPLEMENTATION IN LOW CRUDE PROTEIN DIETS AND THEIR IMPACTS ON GROWTH PERFORMANCE, BODY COMPOSITION, AMINO ACIDS PROFILE AND HEMATOLOGY OF Labeo rohita FINGERLINGS, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 1.
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