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Objective: To determine the antioxidant effects of spinach on the weight of testes and epididymis in obese
Sprague Dawley rats.
Study Design: Experimental, randomized control study.
Place and Duration of Study: It was carried outfrom April 2016 to March 2017 in the department of physiology
of Islamic International Medical College, Rawalpindi, Pakistan.
Materials and Methods: Total 40 male Sprague Dawley rats of about 8 weeks were included. They were
randomly divided into Group A- Fed on standard diet and Experimental group- Fed on high fat diet to induce
th obesity. At the end of 6 week, after inducing obesity, experimental group was subdivided into Group B and
Group C. Weight of the testes and epididymis of Group A and Group B rats was recorded. Then 5% spinach was
given to Group C along with high fat diet for 4 weeks and finally weight of the testes and epididymis was
measured.
Results: Weight of the testes and epididymis (g) of Group B (1.32 ± 0.53g) rats was significantly decreased
(P<0.001) as compared to Group A (1.88 ± 0.92g) rats. However, weight of testes and epididymis (g) of Group C
(1.92 ± 0.49g) rats was significantly increased (P<0.001) as compared to Group B (1.32 ± 0.53g) rats after
spinach intake.
Conclusion: Intake of spinach supplemented diet has ameliorative effects on weight of testes and epididymis in
response to deleterious effects caused by obesity-induced oxidative stress.
Somia Iqbal, Shazia Ali, Arif Siddiqui. (2018) Antioxidant Effects of Spinach (Spinacia Oleracea) on Testicular and Epididymal Weight of Obese Sprague Dawley Rats, , Volume-13, Issue-3.
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