تلخیص
Myzus persicae (Hemiptera, Aphididae) is a widely distributed, devastating and global
sap-sucking crop pest with the diversity of host plants and scratched billions of dollars
economically. Wolbachia is a widespread endosymbiotic bacteria and the present study
was carried out for the first time to determine the phylogenetic relationship erected on
mitochondrial (COI) gene in aphid populations. The screening of Wolbachia was
surveyed by wsp general primers in M. persicae populations. Ten food plants were
selected to study the food preference of the host M. persicae populations in greenhouse
conditions and artificial diets for lab rearing. M. persicae samples were collected from
fifteen geographically distant localities of Pakistan. Eggplant and cabbage revealed
significantly higher inclinations as compared to other host plants (cauliflower, tomato,
sweet potato, Lettuce, broccoli, burdock) whereas carrot and papaya were less preferred
by M. persicae. Comparison between natural and artificial diets exhibited maximum
populations in natural diets in greenhouse conditions as compared to artificial diets in
lab conditions except for June and July. Screening of Wolbachia using PCR markers
revealed positive amplicons in M. Persicae. The infection rate persisted (Punjab
16.29%, Khaber Pakhtunkhawa 6.66% and Sindh 8.88 %) conferred by quantitative
PCR analysis. Retrieved sequences through mitochondrial COI gene were deposited in
gene bank (accession numbers KY509874 and KY522912). The scrutinized dataset
depicted the genetic variation of M. persicae populations. Wolbachia is a conjoint and
rampant throng of the endosymbiotic microbe and may be acknowledged as a possible
means for aphid pest management programs.
Bilal Rasool, Zeeshan Nabi, Muhammad Adnan Bodlah, Naveed Afzal, Khizer Samiullah, Awais Rasool, Rizwan Rasool. (2020) Host food preference, screening and phylogenetic analysis of Wolbachia in Myzus persicae populations, Asian Journal of Agriculture and Biology, Volume 8, Issue-4.
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