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The use of various nanoparticles in industry and domestic practices enviably end into the aquatic environment. Nanoparticles have the potential accumulative and toxic influence in the water environments. There is a need to exploremore likely accumulation of nanoparticles in the aquatic organisms. This study was designed to investigate the possible bioaccumulation of TiO2, CuO and ZnO nanoparticles to tilapia (Oreochromis mossambicus). Fish were exposed to three doses of nanoparticles suspensions T0 (no dose administration), T1 (0.5 mg/L), T2 (1.0 mg/L) and T3 (1.5 mg/L) for 14 days. After 14 days, animals were slaughtered and organs (gills, liver and muscles) were freeze dried. Freeze dried tissue samples were digested with the help of nitric acid (HNO3) and perchloric acid (HClO4). Acid digested samples were analyzed by Inductively Coupled Plasma Mass Spectrophotometry (ICP-MS) foraccumulation of Ti, Cu and Zn nanoparticles in various tissues (gills, liver and muscles). Ti and Cu accumulation in gills was recorded to be 5.7033 and 3.7133 µg/Kg respectively,whereas, maximum Zn accumulation (2375.3 µg/Kg) was observed in liver. It was concluded from the present study that the strategy applied to assess the bioaccumulation of nanoparticles in the soft tissues of fish is very useful tracking method to determine the accretion in aquatic organisms.

Khurram Shahzad, Muhammad Naeem Khan, Farhat Jabeen, Nasreen Kosour, Muhammad Sohail, Muhammad Khalil Ahmad Khan, Munir Ahmad. (2017) Bioaccumulation of manufactured titanium dioxide (TiO2), copper oxide (CuO) and zinc oxides (ZnO) nanoparticles in the soft tissues of tilapia (Oreochromis mossambicus), Punjab University Journal of Zoology, Volume 32, Issue 2.
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