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Motility is a dynamic and remarkable element of bacterial physiology and determines the ability of bacteria to form biofilms. There is a need to control bacterial motility. Present study determined swimming, swarming, and twitching motilities in response to different physiochemical factors. Twenty-two morphologically different bacterial strains were collected from a drain of sewage water located in the vicinity of Jinnah hospital Lahore, Pakistan. Six biofilm forming bacterial strains were selected on the basis of their motility and biofilm forming ability firstly by growing them on congo red agar medium and then by performing ring test. By 16S rRNA sequencing, bacterial strains were identified as Exiguobacterium aurantiacum, Bacillus subtilis, Bacillus megaterium, Bacillus endophyticus, Pseudomonas fragi and Bacillus subtilis. The optimum temperature and pH for swimming, swarming and twitching motilities was 37°C and pH 7. All the bacterial strains showed reduction in diameter of zones of all motilities when the concentration of NaCl increased in medium. Among chemical factors, tobramycin
was proven to have excellent efficiency to reduce bacterial motilites whereas proteinase K enzyme showed weak antimotility effect against all tested bacterial strains. From this study we proposed that instead of biofilm detachment by expensive commercial detergents, we could change the physical environment of sewage systems as well as flooding some specific chemicals, which disrupt bacterial motilities and biofilm formation.
Anjum Nasim Sabr, Siddra Tayyab Akhtar. (2017) Twitching, swimming, swarming in biofilm forming strains in response to chemical and physical factors, Punjab University Journal of Zoology, Volume 32, Issue 2.
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