تلخیص
The objective of the present study was to evaluate the effect of co-inoculations of auxin producing
Bacillus and Streptomyces strains to enhance the growth of Triticum aestivum L. For auxin
production, Bacillus cereus McR-3, B. subtilis McR-7, Enterobacter cloacae FR, Streptomyces
sp. RSF, S. macrosporeus SCF and S. griseoincarnatus CTF were screened by using colorimetric
method. Auxin production was determined in the absence and presence of precursor i.e. Ltryptophan (0, 100, 300, 500 µg ml-I). Highest auxin production was recorded with B. subtilis
McR-7 (31 µg ml-I), B. cereus McR-3 (28 µg ml-I) and E. cloacae (95 µg ml-I), respectively, in the
presence of 100, 500, 500 µg ml-I L-tryptophan. After in vitro auxin screening, strains were
evaluated for their growth stimulatory effects on T. aestivum under axenic conditions. In pot
trials, strains showed variable growth responses when inoculated with single or mixed cultures. In
single cultures, significant increase for root length (49%), shoot length (42%) and shoot fresh
biomass (31%) was recorded respectively with Streptomyces sp. RSF and S. macrosporeus SCF.
For mixed cultures, McR-3+SCF, FR+RSF and McR-7+SCF were the most promising to enhance
shoot length (35%), root length (18%) and shoot fresh biomass (20%), respectively, over control.
Overall, single and a few combinations of mixed cultures were most suitable to enhance growth of
T. aestivum under axenic conditions. Results also indicated that consortia comprising of plant
growth promoting rhizobacteria (PGPR) and Streptomyces offer good potential for the
formulations of bio-inoculants for field trials.
Anam Jamil, Basharat Ali. (2014) L-TRYPTOPHAN AMENDMENTS ENHANCED AUXIN PRODUCTION AND GROWTH OF TRITICUM AESTIVUM L. BY RHIZOBACTERIA, Asian Journal of Agriculture and Biology, Volume 2, Issue 4.
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