تلخیص
Although most of the soils are rich in total phosphorus (P), but its large portion remains unavailable to plants and is considered as a limiting factor for plant growth especially in alkaline calcareous soils. Phosphate solubilizing bacteria (PSB) is a group of bacteria that enhance the P availability by solubilizing hydrolysable inorganic P compounds in addition to mineralization of soil organic P. Sixteen bacterial isolates were isolated from the rhizosphere of mung bean and tested in lab for phosphate solubilization and phosphatase activities. Five bacteria were found positive for P solubilization as well as in vitro production of phosphatase enzyme. A pot experiment was conducted to evaluate the selected five phosphate solubilizing bacteria in combination with poultry manure (PM) on growth and yield of mung bean. Results showed that inoculation with PSB improved the growth and yield of mung bean in presence as well as absence of PM as compared with un-inoculated control. However, PSB isolates were most effective for enhancing yield and growth of mung bean in the presence of PM compared with their use in absence of PM, most probably due to their phosphatase activity. Maximum increases in pods number per plant (80%), 100 grain weight (57%), and grain yield per plant (36%) were observed by combined use of isolate S5 and PM as compared to un-inoculated control without PM. Combined application of PSB and PM also caused significant increase in phosphatase activity of the rhizosphere (79% over control) and available P contents (45% over control) in soil. The most efficient bacterial isolate (S5) was identified as Bacillus thuringiensis on the basis of rrs (16S rRNA) gene sequencing. It is concluded that multi-trait PSB with phosphatase activity in combination with organic amendments could be more convincing to enhance P bioavailability for plants especially in alkaline calcareous soils
Shazia Iqbal, Muhammad Yahya Khan, Hafiz Naeem Asghar, Muhammad Javed Akhtar. (2016) Combined use of phosphate solubilizing bacteria and poultry manure to enhance the growth and yield of mung bean in calcareous soil, The Journal Soil & Environment , Volume 35, Issue 2.
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