تلخیص
Declining population of honeybee day by day is the major threat, and this decline is due
to variety of stressors. As honeybees are considered most important pollinators of our
managed and wild corps, we investigated the effect of neonicotinoids; acetamiprid,
imidacloprid, thiacloprid and clothianidin on honeybees by ingestion and contact method.
Finding of this study showed that among these neonicotinoids, imidacloprid and
thiacloprid were most toxic insecticides with 100% mortality at realistic field doses by
ingestion method with sugar solution as a substitute of nectar after the exposure of 3hrs.
Honeybee’s workers exposed with insecticides mixed pollen grains showed that
imidacloprid and clothianidin were most toxic as compared to other insecticides at
recommended field dose with 100% mortality and minimum mortality with 60% was
observed at thiacloprid field dose after 3hrs exposure. The results of contact toxicity
showed that all four insecticides were most toxic with wet filter paper with 100%
mortality at recommend field dose after the exposure of 3hr, and with dry filter paper
imidacloprid and thiacloprid showed 100% mortality and acetamiprid and clothianidin
with 53.3% mortality after the exposure of 3hrs at field dose. Overall conclusion from
the present study indicates that from these four insecticides imidacloprid was the most
toxic as compared to all others on both ingestion and contact method at recommended
field dose.
Muhammad Imran, Tayyaba Naseem, Arshad Iqbal, Khalid Mahmood, Aslam Sheikh. (2018) Assessment of sensitivity level of honeybee (Apis mellifera) to neonicotinoid insecticides, Asian Journal of Agriculture and Biology, Volume 6, Issue 3.
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