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Bacterial isolates were cultivated in aqueous extracts of watermelon’s rinds, its pulp, peels of mangoes and apples and sugarcane bagasse. The isolate designated as AI-1 showed maximum growth with initial pH 5 at 30°C with 10% of 72 hours old inoculums. The isolate AI-2 and AI-3 grew best at 45¬C and 37¬C with initial pH 9 and 10% 72 hours old inoculum. The isolate AI-4 showed maximum growth at 45°C and initial pH 9 and 10% 72 hours old inoculum. The bacterial isolates AI-5 and AI-6 showed maximum growth at 30¬C with initial pH 9 with 5% and 10% 48 and 24 hours old of inocula size and age, respectively. The isolates AI-2, AI-4, AI-5 and AI-6 were found to be ethanologenic when cultured in batch fermentation for five days. The bacterium AI-6 grew best in aqueous extract of watermelon rinds and sugarcane bagasse and yielded up to 7.4% ethanol at 6th day of fermentation. The reported bacterial diversity can be exploited for conversion of wastes into ethanol and bacterial cell biomass under a range of different physicochemical parameters i.e, pH and temperature. Neutral pH and higher temperature optima for these isolates make them good candidates for obtaining bioethanol from agriwastes in non-aseptic conditions.
Awais Ibrahium, Javed Iqbal Qazi, Muhammad Nadeem, Hafiz Abdullah Shakir. (2014) Potential of cellulolytic bacteria for ethanol production from agri-food wastes, Punjab University Journal of Zoology, Volume 29, Issue 2.
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