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Diatomaceous earth (DE) formulations are being used as grain protectants with reduced environmental issues and insecticide resistance. In this study binary response of 2nd, 4th larval instars and adults of Tribolium castaneum (Herbst) were recorded (1, 2, 5, 7 and 14 days exposure interval) on DE treated (50, 100, 150, 300, 400 and 500 mg/kg) wheat. Mortality percentage for different life stages and F1 Progeny reduction at low and high temperature and relative humidity conditions were observed as efficacy determining tools. About 100% mortality was recorded after 7 day interval at 28±2°C; 55±5% RH with low LC50 and LC90 values in 2nd instar larvae as compared to high LC50 and LC90 values for 4th instar larvae with a maximum of 81.94% mortality at 28±2°C; 55±5% RH. Mortality rates for 2nd instar (70%) and 4th instar larvae (65%) were much lower at 22±2°C; 40±5% RH. Adults of T. castanium appeared to be more tolerant with a maximum of 42.5% response at 28±2°C, 55±5% RH. There was greater than 70% progeny suppression at 400mg/kg dose rate and it was also observed that there was complete progeny suppression (100%) at low temperature and humidity conditions. So, from these results we can conclude that Nature’s Wisdom DE can be used as effective gain protectant and its low silica content (

Qurban Ali. (2021) PUISSANCE OF NATURE’S WISDOM FOOD GRADE DIATOMACEOUS EARTH AGAINST Tribolium castaneum (HERBST); A STEP TOWARDS ECOFRIENDLY PEST MANAGEMENT, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 2.
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