مقالے کی معلومات
جلد
شمارہ
مقالے کی قسم
زبان
تلخیص
Peroxidase, extracted from soybean seeds was isolated by ammonium sulfate precipitation technique and purified
by ion exchange and gel filtration chromatography. The crude enzyme having 17.29 U/mL activity and 1.586 U/mg
specific activity was subjected to ammonium sulfate precipitation technique for partial purification and the resulted
activity and specific activity were 12.85 U/mL and 5.68 U/mg respectively. After ion exchange chromatography
through DEAE-cellulose, fraction No. 43 exhibited maximum activity of 18 U/mL and specific activity of 9.5 U/mg.
This fraction was then applied to sephadex G-75 column and after elution, the activity and specific activity was
enhanced to 16.04 U/mL and 14.948 U/mg respectively.
Key Words: Soybean seeds, peroxidase purification, ion exchange chromatography, gel filtration
Farzana Alyas, M. Anjum Zia. (2002) EXTRACTION AND PURIFICATION OF PEROXIDASE FROM SOYBEAN SEEDS, , Volume 39, Issue 4.
-
Views
303 -
Downloads
48