تلخیص
Serum samples of unmarried young females (N-34) of ages ranging 19-25 years, Were drawn on mid luteal phase (at 7" or 8" day before the next menstruation). Prior to sampling, it was made certain that the subjects were definitely passing through the required phase. In case of irregular cycle, the sampling was made on 21“ day of the menstrual cycle. Different samples were catcyorized into normal regular (27-29 days), short regular (24-26 days), long regular (30-31 days) and irregular evcle. Sodium Dodecyl Sulphate-Polyacryvlamide Gel Electrophoresis (SDS-PAGE) based on the method of Laemmli (1970), was employed for studying the low molecular weight protem fractions in all cycling patterns. The vel photography. image storing and quantification of Various protem fractions were carried out by Gene Genius Brommaging Gel Documentation System that provided the data of molecular welvhts and percent areas covered by each of the fractions. The data was analysed statistically using Student “U test
and emploved in finding the enhancement reduction andor appearance disappearance of particular protein fractions for comparisons among normal, short. long and irregular cycles. Low molecular weight serum protein fractions were ranging between 26-11 kDa in all cycling patterns. Significant protein fractions of 14 and 13 kDa were found to be
declined in short compared to normal regular cycle. Protein fractions of 17. 15 and 13 kDa indicated a pronounced decline in irregular compared to short regular cycle. The. fractions of 17 and 11 kDa indicated a significant elevation in long compared to short regular evele. No significant protein was found in irregular compared to long regular
cyele.
ABDUL MAJEED CHEEMA, NABILA ROOHI, ASMA ABDUL MALIK, ABDUL QAYYUM NAYYER. (2004) SERUM PROTEIN PROFILE IN LUTEAL PHASE OF CYCLING WOMEN, Punjab University Journal of Zoology, Volume 19, Issue .
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