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A simple, specific, accurate and economic reverse phase liquid chromatographic method wasdeveloped forthe estimation of Sofosbuvir in all polymorphic form. Mobile phase containsacetonitrile and 0.05M ammonium acetate (pH 7.6) in ratio of 50:50 (v/v). A logarithmiccalibration curve was plotted from10μgmL-1to60μgmL-1(r= 0.9989) for sofosbuvir, withthelimit of detection (LOD) and the limit of quantification(LOQ)of 0.245μgmL-1and 0.816μgmL-1,respectively. The developed method was highly validated andpracticed for the assurance ofsofosbuvir raw materials with acceptable and non-objectionable accuracy and precision results(recovery 99–102%, RSD <1.2%, n = 3).Zero significant difference (t-test) was obtained betweenthe overall results of the developed RP-HPLC method
Yasir Mehmood, Ikram Ullah Khan, Yasser Shahzad, Syed Haroon Khalid, Sajid Asghar, Abid Mehmood Yousaf, Talib Hussain, Muhammad Irfan, Ikrima Khalid. (2019) A New,Rapid, Cost-Effective, Easy and ValidatedRP-HPLC Method for Determination of Antiviral(Sofosbuvir) in Bulk Forms, Pakistan Journal of Analytical & Environmental Chemistry, Volume 20, Issue 1.
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