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A thermo-tolerant and deoxyglucose-resistant mutant of Saccharomyces cerevisiaewas developed and employed to convert them to fuel ethanol in a 150 litre fermenter. Maximum ethanol production was achieved when fermentation of dextrozyme-treated hydrol was carried out for about 36 hours under optimized fermenting conditions. The maximum specific ethanol production rate (qP), and overall ethanol yield (YP/S) were found to be 2.82 g L-1h-1and 0.49 g/g respectively. Determination of activation energy for cell growth (Ea= 20.8 kJ/mol) and death (Ed = 19.1 kJ/mol) and product formation and inactivation (EP=35.8 kJ/moland Edp= 33.5 kJ/mol) revealed the thermo-stability of the organism for up to 47°C.
Farman Ali Shah, Shaheen Aziz, Hafeez ur Rahman Memon, M.I. Rajoka. (2010) Ethanol Production Kinetics by a Thermo-Tolerant Mutant of Saccharomyces Cerevisiae from Starch Industry Waste (Hydrol), Pakistan Journal of Analytical & Environmental Chemistry, Volume 11, Issue 1.
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