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Blood samples of healthy adult humans (n = 40) and dogs (n = 30) grouped as males and females, were
collected aseptically through appropriate measures. Thin blood smears were stained using Field stain ‘A’ and
‘B’. The PMNs were counted and observed for Barr bodies attached to them. Only the neutrophils with
terminable lobes were examined and counted. The mean value ± SE of the Barr bodies was calculated and the
difference between males and females of both species was analyzed through independent T-test. The mean ±
SE values for Barr bodies was significantly higher (P ≤ 0.05) in human females (7.7 ± 0.63) as compared to their
male counterparts (2.2 ± 0.38). However, statistically non-significant (P ≥ 0.05) result was noticed between
female and male dogs being 1.8 ± 0.32 and 0.8 ± 0.24, respectively. In a nutshell, it is concluded that both the
females of humans and dogs have a higher occurrence of Barr bodies on their PMNs as compared to their male
counterparts. However, sex determination in humans through this cyto-diagnostic technique is quite certain and
precise as compared to that in dogs.
MUSHTAQ HUSSAIN LASHARI, RABIA HAMEED, UMER FAROOQ, MUSADIQ IDRIS, ZIA UR REHMAN. (2018) Sex determination through Barr bodies of neutrophils in Humans and Dogs, Biologia – Journal of Biological Society of Pakistan, Volume 64 (I), Volume 64 (I).
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