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Although there are several screening assays available for screening and
evaluating the presence of biosurfactants but many of these show variable results
under the same conditions and for the same organism. Most commonly applied
screening assays including oil spreading technique (OST), tilted glass slide assay
(TGS), drop collapse assay, emulsification index E24 and emulsification assay
were used to evaluate production of biosurfactants in five biochemically identified
strains isolated form oil contaminated sites. All strains used were known
producers of biosurfactants but showed variable results for different tests. Four of
five strains produced similar results for OST, TGS and emulsification assay. Drop
collapse assay produced negative results when small amounts of biosurfactants
were present. So it was concluded that a single primary screening test alone
cannot identify an organism’s ability to produce biosurfactants and these tests
should be used in combinations to get a reliable picture.
AZKA ASIF, HAREEM MOHSIN, SADIA N CHAUDHRY, WARDA FATIMA. (2017) Comparative Analysis of Biosurfactant Production Assays by Five Indigenous Oil Sludge Bacteria, Biologia – Journal of Biological Society of Pakistan, Volume 63 (II), Issue (II).
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