Abstract
The study aimed to evaluate some agrobiological properties of ctb transgenic
tomatoes under in vivo condition through physiological and biochemical
characteristics relate to fruit yield and quality. Agrobiological parameters are
determined by weighing, measuring and counting. Photosynthetic rate was determined
via uptake carbon dioxide, the spectrophotometric method used to measure
chlorophyll and total carotenoid content, vitamin C was determined using the iodine
titration method, reducing sugar was determined by dinitrosalicylic acid, degree Brix
was measured using an ATAGO N1 refractometer and total acidity in fruit juice was
determined by neutralization method. Study results showed that final harvesting time
for all tomatoes were 150 days including transgenic plants and control. Plant height
(cm) ranged from 80.3 to 83.6, number of compound leaves from 17.6 to 22, and
number of inflorescences from 7.3 to 9.3. The chlorophyll content (mg/g) and the
photosynthetic rate (µM CO2/m2
/s) peaked at young fruit stage in both transgenic
plants and control with values from 0.48 to 0.62 and from 9.08 to 16.77, respectively.
The yield, yield components and fruit shape of transgenic plants and control were also
similar. Number of fruits ranged from 14.6 to 23, fruit weight (g) ranged from 61.5 to
69.3, and individual yield (kg) varied from 0.99 to 1.53. The main biochemical
characteristics of transgenic plants and control were not different, dry matter (%)
accounts for 5.45-5.91, reducing sugar (%) of 1.87-2.22, vitamin C (mg/100 g) of
44.01-46.13, acidity (%) of 0.62-0.89, Brix (%) of 5.23-6.01 and carotene (mg/100 g)
of 3.01-3.84. In conclusion, six ctb transgenic tomato individuals were able to grow
normally under in vivo conditions similar to non-transgenic control plants.
Agrobiological properties between transgenic plants and control were insignificantly
different with p>0.05.
Nguyen Hoang Loc, Le Thi Thinh. (2020) Investigation of agrobiological properties of ctb (cholera toxin B subunit) transgenic tomato under in vivo condition, Asian Journal of Agriculture and Biology, Volume 8, Issue 3.
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