Abstract
The current study looks at acute and sub-lethal Copper toxicity on developing zebrafish
embryos up to 96 hours post fertilisation (PSF). We allocated four-hour PSF zebrafish
embryos in four groups with duplicates, and 500 mg, 50 mg, and 0.05 mg l−1 and a
control group for 96 hours PSF. All fish exposed to 500 mg l−1 copper died within the
first 6 hours PSF. Significant sub-lethal copper toxic effects on developing Zebrafish
were manifested in the 0.05 and the 50 mg groups, by increased heartbeat rate within
the first 25 hours PSF in the 0.05 and 50 mg group. The sub-lethal toxic effect was also
manifested in the metabolism of developing embryos, with a reduced rate of utilisation
of yolk and lower growth rate and anatomical malformations within the first 25 hours
in the 50 mg group and after 50 hours PSF in both the 0.05 and 50 mg group. The results
indicate that reduced metabolic rate and increased heartbeat rate are potential early
signs of sub-lethal Cu toxicity in developing Zebrafish well before other developmental
defects are visually evident
Evangelia Gouva, Cosmas Nathanailides, Ioannis Paschos, Fotini Athanassopoulou, Ioannis S. Pappas. (2020) Reduced metabolic rate and increased heart beat as early signs of sub lethal Copper toxicity in developing Zebrafish, Asian Journal of Agriculture and Biology, Volume 8, Issue 2.
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