Abstract
OBJECTIVE: To determine malondialdehyde, blood lipids and anti oxidants in newly diagnosed
type 2 diabetic patients.
STUDY DESIGN: Observational, comparative study
PLACE & DURATION: Department of Medicine, Liaquat University of Medical and Health
Sciences Hospital from February 2013- May 2014.
SUBJECTS & METHODS: 97 newly diagnosed type 2 diabetics and 50 healthy controls were
selected through non-probability purposive sampling according to inclusion and exclusion
criteria. Blood samples were collected after 8-12 hours of fasting. Fasting blood glucose level,
lipid and lipoprotein levels were measured by Cobas e411 analyzer. Malondialdehyde (MDA),
Superoxide dismutase (SOD) and Glutathione peroxidase (GPX) were measured by Diagnostics
kit. Zinc and ascorbic acid were measured by using Centronic GmbH-Germany Kit. Albumin,
bilirubin, and uric acid were measured on Hitachi Chemistry analyzer. Data was analyzed on
SPSS version 21.0. The significant p-value was taken at ≤ 0.05.
RESULTS: MDA, blood lipids and anti oxidant mechanisms showed significant differences
between diabetics and healthy controls. Total blood lipids and lipid sub fractions were elevated
in diabetics compared to controls. MDA was raised 5.16±0.91 vs. 2.16±0.62 µmol/l in diabetics
and controls respectively (p=0.0001). The SOD, GPX, Ascorbic acid, Zinc, albumin, uric acid and
bilirubin were reduced in diabetics (p<0.001).
CONCLUSION: Diabetes mellitus is characterized by free radical formation, lipid peroxidation,
altered blood lipids and reduced anti oxidant mechanisms. An increase in malondialdehyde and
reduction of antioxidant mechanisms may contribute to secondary complications.
Mona Humaira, Abdul Raqeeb, Azhar Memon, Haji Khan Khoharo. (2016) Malondialdehyde, Blood Lipids and Antioxidant Activity in Newly Diagnosed Type 2 Diabetics, Journal of Liaquat University of Medical and Health Sciences, Volume-15, Issue-2.
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