Abstract
Sacrocephalus latifolius is one of the recipes used in traditional medicine for the treatment of cancer
ailment in South-western Nigeria. This work was aimed at evaluating the extract and fractions of this
plant against human breast (MCF-7) and lung (NCI-H460) cancer cell lines as well as their antioxidant
activity. While the ant-proliferative assay was done using the SRB-assay for the extract (1-250 µg/mL)
and fractions (1-100 µg/mL), the DPPH antioxidant assay was recorded at concentrations between 25-
400 µg/mL. Against the MCF-7 cell lines, the extract and ethyl acetate fractions recorded growthinhibitory activities at all concentrations with GI50 of 73.02 and 63.38 µg/mL. While the chloroform
fraction recorded cytotoxicities of -1.14 and -6.19 % at 50 and 100 µg/mL with GI50 and TGI of 43.18
and 89.70 µg/mL, the hexane and aqueous fractions were inactive. Similar pattern of activity was
recorded against NCI-H460 cell lines with the chloroform fraction exhibiting a higher sensitivity with
GI50 and TGI of 24.12 and 54.50 µg/mL respectively. The antiproliferative activity was further
corroborated by the higher antioxidant activity (IC50 of 17.91 µg/mL), total phenolic (86.30 mg GAE/g)
and flavonoid contents (72.74 mg QUE/g) of the chloroform fraction. The folkloric use of S. latifolius
in the treatment of tumor ailments has been justified by this work.
Ikpefan O. Emmanuel, Ayinde B. Adesina, Ikpefan J. Ohonmoimen, Mudassar Azah, Farooq A. Dar. (2021) In vitro anticancer potential of Sacrocephalus latifolius leaves extract and its fractions on human MCF-7 and NCI-H460 cancer cell lines., Asian Journal of Agriculture and Biology, Volume-09, Issue-2.
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