Abstract
Introduction: Quick intraoperative diagnosis is especially important in CNS tumors where second surgery may
be avoided thus saving patients from many complications. Touch imprint cytology is simple, quick and costeffective method for the rapid diagnosisand is quite applicable to central nervous system tumors. Other methods
for per operative consultation of lesions include squash smear cytology and Frozen section. The drawback of
squash smear technique is that the entire tissue is squashed into the slide and no representative tissue is left for
routine histopathology. Exerting too much pressure on cells badly affect their morphology. Frozen section
requires special and expensive equipment. The rapid freezing causes several artifacts Touch cytology is equally
effective without their associated complications.
Objective: To correlate per operative Touch imprint cytology on brain tumors with conventional histopathology.
Methodology:This was Experimental diagnostic accuracy study with cross sectional time perspective Conducted
in Department of Pathology, Northwest General Hospital and Research Center Peshawar. Fresh brain and spinal
cord tumors including 2 non neoplastic specimens were obtained. Before putting them in the formalin for routine
surgical pathology examination, touch imprint cytology smear was prepared by gently touching the freshly
removed lesion on glass slide at several places. Half of the slides were air dried and half of them were fixed in
100% alcohol. Air dried slides were stained with Diff Quick stain and alcohol fixed slides were stained with
hematoxylin and eosin. These slides were immediately stained under microscope and diagnosis was given.
Results: All the smears were quite cellular; the nuclear details were excellent. The samples were of divergent
nature. Breakdown of the 28 samples was as follows: meningioma (8), astrocytoma (3), primitive neuro-ductal
tumors (2), pituitary adenoma (2), Schwannoma (2), arachnoid cyst (1), colloid cyst (1), dermoid cyst (1),
medulloblastoma (1), glioblastoma multiforme (1), metastatic clear cell carcinoma (1), teratoma (1), benign
vascular lesion (1),Leukemia/ Lymphoma (1), and Inflammatory lesion, spine(1). The finding of all these cases on
touch cytology smear were same except in one case where Schwannoma, a benign peripheral nerve sheath tumor
was diagnosed as other related benign peripheral nerve sheath tumor i.e. neurofibroma. There was 100 percent
accuracy in terms of benign versus malignant and 96 percent accuracy in terms of precise diagnosis.
Conclusion: The touch cytology smear cells were “fresh from oven” abundant and were free from artifacts. Their
morphological details were absolutely preserved. Touch cytology is simple useful technique to get almost
immediate diagnosis on CNS neoplastic and non-neoplastictumors; which may be used alone or complimentary
to frozen section where quick diagnosis is needed.
Tania Khattak, KhalidJavaid, Anwar Ul Haque, Farooq Azam, Tariq Khan. (2020) Diagnostic Accuracy of Touch Cytology of Central Nervous System Lesions, International Journal of Pathology, Volume-18, Issue-1.
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