Abstract
This paper covers the progress made in research on microbial alpha-amylase, a highly demanded
industrial enzyme in various sectors such as food, pharmaceuticals, textiles, detergents, etc.
Amylases are of ubiquitous occurrence and hold the maximum market share of enzyme sales. The
article surveys the a-amylase family and the major characteristics, microbial sources, production
aspects, downstream processing, salient biochemical properties, industrial applications, enzyme
engineering and some recent research developments.
The present study was concerned with the production of αamylase by Bacillus Subtilis. The
fermentation was carried out by 250 mL Erlenmeyer flask. The maximum production of enzyme
optimized at the pH 7.5 (561 IU/mL/min), while the incubation temperature investigated and the
maximum production optimized at 40ºC (553 IU/mL/min), The production of the enzyme was
obtained maximum at 48 hours after incubation (542 IU/mL/min). The nitrogen sources tested
NaNo3 where the best inorganic sources respectively.
Naqibullah, Agha Mohammad Raza, Dost Mohammad Baloch, Mohammad Saeed, Arif Awan, Farhat Abbas, Wadood Kakar. (2011) EFFECT OF DIFFERENT PARAMETERS ON OPTIMUM PRODUCTION OF MICROBIAL ALPHA AMYLASE PRODUCTION FROM BACILLUS SUBTILLUS, Journal of Applied and Emerging Sciences, Volume 2, Issue 1.
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