Abstract
Based on safety and sound immunological principles for animal vaccines, there arises a need for
identifying rational standards for selection of different adjuvant formulations. Montanide ISA206
(W/O/W) was used in this study to prepare the vaccine and was compared with already available
paraffin oil and alum adjuvanted hemorrhagic septicemia vaccines. All the three vaccines were
prepared according to the standard procedures keeping the antigen amount same in all the three
vaccine formulations. The goal of the study was to ascertain immune response in rabbits against
several Pasteurella multocida vaccine preparations including Montanide adjuvanted, paraffin oil
based and alum-precipitated vaccines. Forty male adult rabbits were divided into four groups namely
A, B, C and D and kept in separate cages. Rabbits in groups A, B and C were vaccinated via
subcutaneous route with 0.3ml dose of Montanide vaccine, oil based vaccine and alum-precipitated
vaccine respectively. A booster dose of 0.3 ml of same preparation was given 15 days postvaccination. Rabbits in group D were kept unvaccinated as negative control. All rabbits were bled
pre-vaccination and then every 7 days post-vaccination for nine weeks and sera were subjected to
indirect haemagglutination assay (IHA) and geometric mean titres of anti-Pasteurella multocida
antibodies were determined. The mean and standard errors were also determined. Results showed that
Montanide ISA206 (W/O/W) adjuvanted vaccine gave higher antibody titres as compared with oil
based and alum-precipitated vaccines.
Mohi-ud-din, Mudassar, Mudasser, Habib, Zahid, Iqbal, Iftikhar, Hussain. (2014) IMMUNE RESPONSE OF RABBITS TO HEMORRHAGIC SEPTICEMIA VACCINE FORMULATIONS ADJUVANTED WITH MONTANIDE ISA-206, PARAFFIN OIL AND ALUM, Asian Journal of Agriculture and Biology, Volume 2, Issue 2.
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