Abstract
Phosphorus is one of the major plant growth limiting nutrients and in Pakistan its deficiency found in
90% soils due to alkaline calcareous nature of soils. Plant growth promoting rhizobacteria are
efficient in supplying nutrients to plants by solubilizing the unavailable/insoluble (P) in soil or by
promoting extensive root growth through 1-aminocyclopropane-1-carboxylate (ACC-deaminase)
activity. This study was conducted to assess the effects of phosphate-soluibilizing rhizobacteria and
their impact on wheat growth under axenic conditions. Initially rhizobacteria (S1 to S10) were
isolated through enrichment by rock phosphate [Ca3 (PO4)2] and ACC as sole P and N source,
respecitively. After isolation, identification and characterization of these strains was done for root
colonization, P-solubilization, in vitro ACC-deminase ctivity, auxin production and chitinase activity.
The results revealed that rhizobacterial isolate S8 was best strain and showed 2.6 fold higher fresh
weight, 2.7 fold higher shoot length and 4 fold higher root length of wheat over uninoculated control.
Similarly, S8 was best root colonizer in wheat rhizosphere (8.0 x 10-7 cfu g-1) while isolate S9 showed
the highest P- solubilization i.e. 14.36 ppm, S2 showed maximum ACC-deaminase activity (1.8 µmol
α-ketobutyrate) and S4 showed highest auxin production (36.77 mgL-1). This study concluded that S8
strain efficiently solubilize phosphate alongwith ACC-deaminase trait which improved the growth of
wheat under axenic conditions.
Shabana Ehsan, Ifra Saleem, Hafsa Zafar. (2014) EFFECT OF PHOSPHATE SOLUBILIZING BACTERIA ALONG WITH ACCDEAMINASE ON WHEAT GROWTH UNDER AXENIC CONDITIONS, Asian Journal of Agriculture and Biology, Volume 2, Issue 3.
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