Abstract
This study was focused on isolation and biochemical characterization of pea (Pisum sativum L.) seed
borne bacteria. A total of 25 bacteria were isolated from pea seeds collected from different sources.
Of 25 isolates, 3 most aggressive isolates (Psp-1, Psp-6, Psp-14) which produced hypersensitive
response (HR) within 24 hours of post inoculation on tobacco leaf were picked for further biopathological assays. Bacterial isolates were able to produce brown necrotic spots on pea leaves after
few days of inoculation which later coalesced and gave peculiar blight like appearance. However,
bacterial isolates were unable to reduce nitrogen and also unable to grow at 41 0C which considered as
a hall mark of only plant pathogenic pseudomonads. Furthermore, results of LOPAT (levan
production from sucrose (L), presence of oxidase (O), pectolytic activity on potato (P), the presence
of arginine dihydrolase (A) confirmed that seed borne bacteria were Pseudomonas syringae pv pisi
which are reported as looming threat to pea production in different parts of world. Therefore, current
study gave a clue about the presence of potential bacterial pathogens in seeds which would easily be
resulted into future disease outbreak. Thus, regular disease surveillance and pathogenicity assays on
commercial pea varieties must be incorporated to avoid any future pea epidemic.
Komal Zafar, Mehmood Ul Hasan, Muhammad Waseem Ali, Muhammad Usman Raja, Gulshan Irshad, Farid Asif Shaheen. (2015) ISOLATION AND CHARACTERIZATION OF SEEDBORNE PSEUDOMONAS SYRINGAE PV PISI FROM PEA (PISUM SATIVUM L.), Asian Journal of Agriculture and Biology, Volume 3, Issue 3.
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