Abstract
In effort to develop bacterial based biofungicide, a rhizobacterium Serratia plymuthica
UBCR_12 showing antifungal activity against phytopathogen Colletotrichum
gloeosporioides was successfully isolated. Prior to its mass application its safety aspect
to the human being and environmental factors has to be characterized in detail as well
as its efficacy. For this purpose, biochemical characterizations of the isolate involving
chitinolytic activity assay based on colloidal chitin degradation, siderophore
production assay using CAS medium, and hemolytic test to identify its pathogenicity
potential was performed. Its antifungal activity based on its capability to inhibit
mycelial growth of fungus finally was determined. The results showed that optimal
chitinolytic activity (1.63 ± 0.15) of the isolate was at pH 7.0, temperature 30 ˚C. The
isolate showed a typical response to substrate concentration 2% (w/v) of colloidal
chitin. Furthermore, the isolate showed a positive reaction with CAS medium and
could lyse red blood cell on the blood agar. This result will imply to mass production
and future application of S. plymuthica UBCR_12 as a biocontrol agent for C.
gloeosporioides. Hence precaution to the human health and environmental safety has
to be taken into consideration for its application.
Jamsari Jamsari, Rahmi Kamelia, Sumaryati Syukur, Lily Syukriani, Istino Ferita. (2018) Biochemical and pathogenic potential characterization of Serratia plymuthica UBCR_12 as promising biological agents for controlling Colletotrichum gloeosporioides, Asian Journal of Agriculture and Biology, Volume 6, Issue 1.
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