Abstract
Liriomyza sp. leafminer fly is an important pest of shallot crops that spread widely in
Southeast Asia and Pacific regions such as Indonesia, China, Japan, Korea, Thailand,
Malaysia, Singapore, Taiwan and Vietnam. This study aims to identify the type of
Liriomyza species attacking onion plants in Bali, Indonesia through morphological and
molecular characterization. Onion leaf samples showing symptoms of Liriomyza attack,
taken from the study site, were subsequently cultured in the laboratory. Adult flies
obtained from breeding were then identified based on their morphological
characteristics. While the molecular characteristics were identified through several
steps: DNA isolation, DNA electrophoresis, PCR chain reaction, then put back into the
re-electrophoresis box, and finally sequencing method was applied. The results showed
that the Liriomyza species was found to have a morphological characteristic such that
it has a simple color pattern on a simple abdomen with no connecting line between the
segments and the next segment, dark grey or near black scutellum. These characteristics
refer to the species L. chinensis. GenBank data tracking results through identification
of squamous alignment results indicate that the Liriomyza COI nucleic acid found
showed the highest homology with L. chinensis (EF104701.1), L. chinensis
(AB721340.1) and L. chinensis (AB721343.1) with identical rate of 99%. Based on the
results then Liriomyza species found attacking onion planting on the island of Bali is L.
chinensis.
Hamid, Wayan Supartha, Wayan Susila, Putu Sudiarta. (2018) Morphological and molecular characteristics of Liriomyza sp. (Diptera: Agromyzidae) on onion plants (Allium cepa L.) in Bali, Asian Journal of Agriculture and Biology, Volume 6, Issue 4.
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