Abstract
Toxic effects of pesticides are diverse. Indiscriminate and extensive use of pesticides has exerted enormous pressure on the environment.Target organisms have developed resistance, whereas many microorganisms have developed detoxification potential too. In this study, three isolates of Pseudomonas i.e., P. aeruginosa, P. aeruginosa MY06 and P. aeruginosa SWD were employed for degradation of malathion. Media containing different concentrations of malathion, as sole source of carbon were inoculated with the bacteria. The cell free fluids of 192 hours old cultures were then injected into the fertilized eggs of Gallus domesticus on third day of incubation and the embryos were recovered on day 7. Non-remediated insecticide preparations induced dose-dependent developmental abnormalities in chick embryos. Whereas cell free culture fluids could produce developmental defects of lesser severity as assessed by morphological and morphometric parameters. Remediated group embryos differed significantly from non-remediated, showing far less drastic effects of the bacterially degraded malathion. The present study indicates bioremediation of malathion with P. aeruginosa, P. aeruginosa MY06 and P. aeruginosa SWD, reduced its toxicity to a significant extent. Screening of the cell-free culture fluids forembryotoxicity, provides an easily workable in ovo toxicity evaluation model.
Shagufta Andleeb, Asmatullahand JavedIqbalQazi. (2014) In-ovo evaluation of bioremediated malathion, Punjab University Journal of Zoology, Volume 29, Issue 1.
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