Abstract
The organisms which show potential to survive and work in acidic and alkaline environment play an important role in various industrial processes. This study was carried out to screen, isolate and characterize alkaliphilic bacteria from industrial wastes. For this purpose
water and soil samples were collected from Chishtian and Quaid-e-Azam industrial area of Kott Lakh-Patt, Lahore. Screening of bacteria was carried out on LB agar plates at pH 8, 9 and 10. Out of 39 isolates, 20 were found to be alkaliphilic in nature. Glycerin stocks were prepared and they were characterized physically and biochemically for identification which revealed that they belong to genera Alcaligenes, Escherichia, Natronobacterium, Aeromonas, Pseudomonas, Marinococcus, Neisseria, Micrococcus, Sporosarcina, Pleisomonas and Cupriavidus. BCTL-147 isolate growing at pH 10, belonging to genus Alcaligenes was further characterized for optimum growth conditions (temperature and pH). The optimum pH was found to be 10 while optimum temperature for growth was 35°C. The growth curve of this isolate was prepared at optimum conditions. This high optimum pH bacterial isolate can further be tested for its biochemical potential and other significant properties.
SYED SHAHID ALI, IFFAT HABIB , TANZEELA RIAZ. (2009) SCREENING AND CHARACTERIZATION OF ALKALIPHILIC BACTERIA FROM INDUSTRIAL EFFLUENTS , Punjab University Journal of Zoology, Volume 24, Issue (1&2).
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