Abstract
Microorganisms like staphylococci and pseudomonads are responsible for food poisoning. These are natural residents of soil and water and cause primary skin infections. In present study, antibiotics were used against these bacteria through diffusion disc method. Two types of
media (MacConky agar and nutrient agar) were used. Pseudomonas
syringae was found to be sensitive to tobramycin, ciprofloxacin, and
ampicillin while it was resistant to tetracycline, gentamycin, penicillin-G,
kanamycin, and streptomycin. Staphylococcus aureus was sensitive to
kanamycin, gentamicin, tobramycin, streptomycin, and ampicillin, while
it was susceptible to the various selected antibiotics such as ciprofloxacin, tetracycline, and amoxicillin. Genomic DNA was extracted to detect the defense genes both from P. syringae and S. aureus through modified techniques for amplification of various genes which play some role in prevention of various related diseases
MARIAM JAMIL, SAIQA ANDLEEB, SHAUKAT ALI. (2009) ANTIBACTERIAL ACTIVITY OF ANTIBIOTICS AGAINST PATHOGENS AND THEIR GENOMIC DNA ISOLATION, Punjab University Journal of Zoology, Volume 24, Issue (1&2).
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