Abstract
The ability to isolate clean, intact RNA has important uses in cloning genes and is essential to analyze gene expression. To get useful
clones, it is critical that full-length RNA is used as the starting material.
To obtain good quality of eukaryotic mRNA it is necessary to minimize
the activity of ribonuclease liberated during cell lysis by using their
inhibitors. Guanidinium thiocyanate and 2-mercaptoethanol containing
buffer ensures thorough denaturing of macromolecules and inactivation
of RNases. Many different protocols and commercial kits are available
for isolation of RNA from tissue but all have their own limitations. A
single step isolation method for RNA was modified to get good quality
total RNA from RNases rich tissue e.g., pancreas. Tissue samples (chick
and bovine pancreas) were collected under RNases free conditions. Total RNA was extracted using guanidinium thiocyanate and acid phenol. Two discrete bands of 28S and 18S rRNA were appeared on formaldehyde agarose gel that suggest isolation of good quality intact RNA.
NAAZ ABBAS, ZAINAB ABBAS , M; AKHTAR. (2006) AN IMPROVED METHOD FOR THE EXTRACTION OF TOTAL RNA FROM RIBONUCLEASE RICH TISSUES, Punjab University Journal of Zoology, Volume 21, Issue (1&2).
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