Abstract
A comparative study of total seed protein profile was carried out to determine the extent of genetic variability amongst 53 genotypes of Indian mustard [(Brassica juncea L.) germplasm using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) technique. Total seed protein was estimated. To estimate genetic diversity, 12 different types of protein bands were obtained based on the banding pattern of all the genotypes; seven (58%) were polymorphic and five were monomorphic. The protein size base polymorphism revealed the range of protein bands based on their molecular weights which ranged from ~10 kDa to ~180 kDa. Dendrogram was constructed using UPGMA method which divided the genotypes into five groups: group I had 18 genotypes, group II 17 genotypes, group III 3 genotypes, group IV 13 genotypes and group V 2 genotypes. Among the studied genotypes, similarity coefficient values ranged from 17% to 100%. Highest similarity coefficient (i.e., 100%) was recorded between Bj765 and Bj785, Bj790 and Bj791, Bj790 and Bj794. The observed clusters based on this study exhibited a genetic diversity among these genotypes of Indian mustard on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) level. Thus, this study revealed that the SDS-PAGE method plays a key role in the study of protein based variation among different genotypes of plant species.

Fida Muhammad Abbasi, Malik Ashiq Rabban, Inamullah, Sohail Ahmad Jan, Muhammad Ilyas, Haris Khurshid, Muhammad Ishaq Ibrahim. (2017) Evaluation of Genetic Variation among Indian Mustard (Brassica juncea L.) Genotypes by SDS-PAGE Method, , Proc. of the PAS: B; 54, Issue 4.
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