Abstract
A comparative study of total seed protein profile was carried out to determine the extent of genetic variability amongst 53 genotypes of Indian mustard [(Brassica juncea L.) germplasm using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) technique. Total seed protein was estimated. To estimate genetic diversity, 12 different types of protein bands were obtained based on the banding pattern of all the genotypes; seven (58%) were polymorphic and five were monomorphic. The protein size base polymorphism revealed the range of protein bands based on their molecular weights which ranged from ~10 kDa to ~180 kDa. Dendrogram was constructed using UPGMA method which divided the genotypes into five groups: group I had 18 genotypes, group II 17 genotypes, group III 3 genotypes, group IV 13 genotypes and group V 2 genotypes. Among the studied genotypes, similarity coefficient values ranged from 17% to 100%. Highest similarity coefficient (i.e., 100%) was recorded between Bj765 and Bj785, Bj790 and Bj791, Bj790 and Bj794. The observed clusters based on this study exhibited a genetic diversity among these genotypes of Indian mustard on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) level. Thus, this study revealed that the SDS-PAGE method plays a key role in the study of protein based variation among different genotypes of plant species.