Abstract
Clinical facility for present study was available at Al-Bashir Hospital Sadiqabad. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) based on the method of Laemmli (1970) was employed for studying the low molecular weight serum protein fractions of affected and control families. Gel photography and quantification of various protein fractions were carried out by Gene Genius Bio-imaging Gel Documentation system that provided the data of molecular weights and percent area covered by each of fractions. Data was employed in finding the enhancement or reduction and appearance or disappearance of particular protein fraction for comparison between affected and control families. Six protein fractions were detected in control families. Protein fraction of llkDa was detected only in affected families and could not be expressed in controls. Protein fractions of 17kDa and 20kDa exhibited significant decreases in affected families when compared to control families. Protein fraction of 14kDa that appeared in control was absent in most of the affected families.
NABILA ROOHI, ABDUL MAJEED CHEEMA , ASMA SIDDIQUE. (2002) Investigation into Familial Relationship in Protein Profile in Kidney Stone Disease, Punjab University Journal of Zoology, Volume 17, Issue.
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