Abstract
Serum samples of healthy women and hyperthyroid patients were obtained alongwith the radioimmunoassay values of triiodothyronine, thyroxine and thyroid stimulating hormone. Samples were diluted in phosphate buffer (pH 7.2) and proteins were denatured by heating with loading dye. SDS-PAGE was employed for studying the protein profile of healthy and hyper thyroid subjects. Gels were photographed and their images were stored for quantification of protein fractions by UVP Gel Base Software Programme that provides the data of molecular weights and percent areas covered by each of the fractions. Data was employed in finding the enhancement or reduction and the appearance or disappearance of particular protein fractions for comparison between healthy and hyperthyroid groups. A total of 21 fractions were detected in normal subjects, all of which were expressed in hyperthyroid group. Most of the fractions in hyperthyroid group were found to be markedly reduced when compared to healthy group. No new fractions were encountered in hyperthyroid compared to healthy group.
NABILA ROOHI, ABDUL MAJEED CHEEMA, SAIMA MUNIR, MUHAMMAD WAHEED AKHTAR. (1998) SERUM PROTEIN FRACTIONS IN HYPERTHYROID WOMEN , Punjab University Journal of Zoology, Volume 13, Issue 1.
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