Abstract
Agrobacterium tumefaciens was isolated from two sources. One source was the young and freshly cut tumors from trees of Populus sp. present along the canal near the vicinity of Quaid-e-Azam Campus, I'unjab University, Lahore. The other source was from stink bugs which were always found to be present in the proximity of the tumors of these trees. These insects habitually feed on them. Total of eight isolates were obtained and designated as F3o, F3w, F3y1, F3y2, F3y2(1), BS, BSc and BSy. First five were, obtained from the tumors and later three were isolated from hemolymph of stink bug. Two of these strains i.e., F3o and BSy were selected for inoculation studies. These were injected as well as fed in measured dozes to the insects. The effects were observed after 5 hrs, as bacteria-treated insects started showing high rate of mortality after 8 hrs. The insects which received bacteria by means of feeding survived for comparatively longer duration. Blood smear preparations of inoculated insects showed a disturbed hemocyte structure. Histological studies of the gut of bacteria-fed insects revealed a significant decrease in the length of the cells of enteric epithelium. Their total body protein content also showed a significant increase over that of the controls, but no significant increase was noted in two case of inoculated insects
FOUZIA QAMAR , FIRDAUSIA AZAM ALI , SHAHIDA HASNAIN. (1991) AGROBACTERIUM TUMEFACIENS AFFECTING SURVIVAL, HISTOLOGY AND PROTEIN CONTENT OF PERIPLANETA AMERICANA (COCKROACH) , Punjab University Journal of Zoology, Volume 6, Issue 1.
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