Abstract
Three cobalt resistant bacteria were isolated from the industrial waste water and were designated as SCo-1, SCo-2 SCo-3. They could resist CoC12 upto 300ug/ml. Colonies of these isolates were circular with entire margins and convex elevation. Colour of Colonies were off white (SCo-I), light brown (SCo-2) and creamy (SCo-3). They had motile rod shaped cells. All were aerobic, had oxidase and catalase enzymes. They were non spore former and were able to produce pigments. All of them shared almost all biochemical characters. On the basis of morphological and biochemical characters these isolates could be affiliated to family pseudomonadaceae. These strains showed optimum pH range 6-8.5 for their growth. They could also bear Zn, Sn, Mn, Cd and Pb (200ug/m1) in the medium. All isolates showed resistance to ampicillin (30Oug/m1) and chloramphenicol (Sug/ml), while they were sensitive to streptomycin, kanamycin and tetracycline. Gel electrophoresis of total cell lysates of these bacteria revealed that only SCo-1 harbour a single plasmid. No transconjugant on CoCl2 + Sm plates were attained. However the yield of large number of transformants on plates amended with CoC12 revealed that cobalt resistance in SCo-1 was encoded by plasmid
SAMINA YASMIN , SHAHIDA HASNAIN. (1991) COBALT - RESISTANT PSEUDOMONADS FROM INDUSTRIAL EFFLUENT, Punjab University Journal of Zoology, Volume 6, Issue 1.
-
Views
395 -
Downloads
42
Previous Article
