Abstract
This study develops a gas chromatographic method coupled to micro-electron capture detector todetermine four basic polychlorinated dibenzo-p-dioxins (PCDDs) congeners: 1, 1,2,3,7,8-pentachlorodibenzo-p-dioxin; 2,2,3,7,8-tetrachlorodibenzo-p-dioxin; 3, 1,2,3,6,7,8-hexachloro-dibenzo-p-dioxin; and 4, 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin in wheat and rice. ThePCDDs were extracted using 1:1 acetone:n-hexane mixture followed by cleaning with acidicaluminium oxide in polypropylene mini columns and eluted with dichloromethane. In quantitativedeterminations, the limit of detection for congener 1 of PCDDs was 0.4 ng mL-1while for othercongeners (2–4) it was found to be 1.0 ng mL-1. The congener 1 was checked atspiking levels of0.02, 0.05 and 0.1 ng g-1, and its recovery was 85.96–120.74% and 95.32–116.88% from wheatand rice, respectively. Wheat and rice were also spiked by congers 2–4 at spiking levels of 0.05,0.1 and 0.5 ng g-1; the recovery ranges from wheat were 87.70–115.54%, 85.64–117.88% and88.40–119.32% for congener 2, 3 and 4, respectively, while from rice the recovery was 77.67–115.68%, 83.18–119.68% and 79.76–131.15% for congener 2, 3 and 4, respectively. The limit ofquantification was determined as 0.1ng g-1for congener 1 and 0.5ng g-1for other three PCDDs(2–4). The intra-day and inter-day RSDs of peak areas (n= 3) for four congeners (2 ng mL-1) wereranged at 2.5–8.1% and 3.1–10.6%, respectively. This study provides a simple and cost-effectivegas chromatographic-electron capture detector method to study some basic PCDDs in wheat andrice grains first time in Pakistan with fair precision and accuracy when expensive high resolutiongas chromatographic-mass spectrometry method is not accessible
Iffat Abdul Tawab Khan, Qurrat-ul-Ain, Zahida Tasneem Maqsood. (2019) Development of Gas Chromatographic Method with ElectronCapture Detector for Determination of Some PCDDs inWheat and Rice Grain Matrix, Pakistan Journal of Analytical & Environmental Chemistry, Volume 20, Issue 1.
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