Abstract
The maximum ethanol production and β-fructofuranocidase formation under fermentation studies were carried out in microprocessor controlled 23-L stainless steel fermenter at the following conditions: Temp = 40 ̊C (wild &mutant organism) , pH= 5.5, carbon source (molasses=15% sugar) nitrogen source (ammonium sulphate 0.75%), 300 RPM stirring speed and oxygen flow rate was 0.1 vvm. Mutant. Strain of thermotolerant Kluyveromyces marxianusM15 produced maximum production of ethanol at 48hr.All Kinetic Parameters have been studied for the utilization of substrate and production of maximum ethanol for both wild and mutant strains. It has been observed that wild strain was growing up to 550C while the mutant strain was growing up to 650C. In this comparison study, wild and mutant strain showedthat Mutant-derived M15 wasstronger over its parental culture due to its more thermal stability and production of ethanol at 65 oC at which wild organism could not grow
Shaheen Aziz, Hafeez ur RehmanMemon, Farman Ali Shah, M.I. Rajoka, Suhail A. Soomro. (2009) Productionof Ethanolby Indigenous Wild and Mutant Strain of ThermotolerantKluyveromyces MarxianusUnder Optimized Fermentation Conditions, Pakistan Journal of Analytical & Environmental Chemistry, Volume 10, Issue 1-2.
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