Abstract
Bacillus thuringiensis (Bt) is a bacterium that can be used as substitute
for various chemical compounds especially insecticides. The insecticidal
property of B. thuringiensis comes from the production of Crystal protein.
The present study is focused on the cloning and characterization of
Cry1AB gene which expresses to produce crystal protein. Cry1AB gene
was amplified using specific primers which gave successful PCR results
of 3.5 kb long gene amplification. The amplified gene was further verified
by inserting it into the expression vector. Furthermore, a computational
approach was used to understand evolutionary relationships among
Cry1AB gene and to identify conserved protein domains in Cry1AB
proteins. Use of insilico approach provided new insights of
understanding functional characteristics of Cry1AB gene and its
variants
AMBASH RIAZ, SHAHID RAZA, HIRA MUBEEN. (2019) Identification and cloning of Cry1AB gene from Bacillus thuringiensis, Biologia – Journal of Biological Society of Pakistan, Volume 65 (I), Issue 1.
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