Abstract
The current study was designed to establish the specie identification of
the predominant Siphonaria species (S. asghar, S. belcheri and S.
kurracheensis) via PCR based amplification of the two mitochondrial
housekeeping genes i.e. 16S rDNA, COI, and one nuclear H3 gene. The
nutritional significance of the Siphonaria sp. is also highlighted via amino
acid profiling. Sampling was conducted at Buleji, the major coastal site of
Karachi which harbours a huge and varied number of gastropods. The
body muscle was dissected out of the shell and used for molecular
identification and biochemical analysis (Amino acid profiling). Macro/micro
morphology of the shells was also studied by scanning electron
microscopy and energy dispersive X-ray spectrometry. The analysis of
nutritional status of the three Siphonaria sp. revealed quite similar profiles
among the three Siphonaria sp with the presence of nine essential and
nine non-essential amino acids. Our results successfully established the
method of molecular identification of Siphonaria sp. The study also
showed that Siphonaria sp. are nutritionally rich and thus can be
consumed as food or feed as valuable marine resource to overcome the
malnutrition problem in the developing countries
FOZIA HUMAYUN , ZARRIEN AYUB, MUHAMMAD SAMEE HAIDER, SYED ABID ALI. (2019) Morphological identification, molecular bar-coding and nutritional value of Siphonaria species from Sindh Coast, Pakistan, Biologia – Journal of Biological Society of Pakistan, Volume 65 (I), Issue 1.
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